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OriGene scramble control
Scramble Control, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcas+guide+crispra/pCas-Guide-Puro-CRISPRa-Scramble/pm41817448-254-13-15
Average 94 stars, based on 1 article reviews

scramble control - by Bioz Stars, 2026-10

94/100 stars

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Plasmid Preparation:

Article Title: Tailoring Magnetite-Nanoparticle-Based Nanocarriers for Gene Delivery: Exploiting CRISPRa Potential in Reducing Conditions.
Article Snippet: Dulbecco’s Modified Eagle Medium (DMEM) was purchased from Gibco (Amarillo, TX, USA), and Fetal Bovine Serum (FBS) was purchased from BioWest (Riverside, MO, USA). .. Primers, sgRNA, the thiolated tag (used to bind the nanobioconjugate to the linearized plasmid), AgeI-HF Enzyme (NEB-R3552S), EcoRI-HF Enzyme (NEB-R3101S), PvuI-HF Enzyme (NEB-R3150S), Q5 High-Fidelity 2X Mastermix (NEB-M0515), Monarch PCR & DNA Cleanup Kit (NEB-T1030S), and Monarch Plasmid Miniprep kit (NEB-T1010S) were obtained from New England Bio labs (Ipswich, MA, USA). pCas-guide-CRISPRa (#GE100055) and pCas-Enhancer (#GE100056) were obtained from Origene (Rockville, MD, USA). .. Cell characterization was conducted in human primary dermal fibroblasts (HDFa, PCS-201-012) purchased from ATCC (St Cloud, MN, USA), and human astrocytes (NHA) were obtained from Lonza (Basel, Switzerland).

Article Title: Tailoring Magnetite-Nanoparticle-Based Nanocarriers for Gene Delivery: Exploiting CRISPRa Potential in Reducing Conditions
Article Snippet: Dulbecco’s Modified Eagle Medium (DMEM) was purchased from Gibco (Amarillo, TX, USA), and Fetal Bovine Serum (FBS) was purchased from BioWest (Riverside, MO, USA). .. Primers, sgRNA, the thiolated tag (used to bind the nanobioconjugate to the linearized plasmid), AgeI-HF Enzyme (NEB-R3552S), EcoRI-HF Enzyme (NEB-R3101S), PvuI-HF Enzyme (NEB-R3150S), Q5 High-Fidelity 2X Mastermix (NEB-M0515), Monarch PCR & DNA Cleanup Kit (NEB-T1030S), and Monarch Plasmid Miniprep kit (NEB-T1010S) were obtained from New England Bio labs (Ipswich, MA, USA). pCas-guide-CRISPRa (#GE100055) and pCas-Enhancer (#GE100056) were obtained from Origene (Rockville, MD, USA). .. Cell characterization was conducted in human primary dermal fibroblasts (HDFa, PCS-201-012) purchased from ATCC (St Cloud, MN, USA), and human astrocytes (NHA) were obtained from Lonza (Basel, Switzerland).

Article Title: Assessment of CRISPRa-mediated gdnf overexpression in an In vitro Parkinson’s disease model
Article Snippet: Buforin II (BUF-II-TRSSRAGLQFPVGRVHRLLRK) was synthesized by GL Biochem Shanghai (Shanghai, China). .. Primers, sgRNA, thiolated tag, EcoRI-HF Enzyme (NEB-R3101S), PvuI-HF Enzyme (NEB-R3150S), Q5 High-Fidelity 2X Mastermix (NEB-M0515), Monarch PCR & DNA Cleanup Kit (NEB-T1030S), and Monarch Plasmid Miniprep kit (NEB-T1010S) were obtained from New England Biolabs (Ipswich, MA, United States). pCas-guide-CRISPRa (#GE100055) and pCas-Enhancer (#GE100056) were obtained from Origene (Rockville, MD, United States). ..

Polymerase Chain Reaction:

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Down-regulation of MOV10 (Moloney Leukemia Virus 10) enhances Hepatitis B virus (HBV) gene expression in HepAD38 cells. ( A ) Time course of the experiment. HepAD38 cells were transfected with 50 nM siMOV10 or siControl 24 h before tetracycline (tet) removal. Cells were collected two days after tet removal. ( B ) Western blot showing HBV core and MOV10 expression in siRNA treated cells. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a loading control. ( C ) Quantification of HBV core expression determined by western blotting. ( D ) Intracellular HBV pgRNA level determined by RT-qPCR. Data were normalized to GAPDH mRNA expression. ( E ) Intracellular HBV DNA level determined by qPCR. Data represent mean ± standard deviation from two independent experiments. * p -value < 0.05, two-tailed unpaired t -test.

Journal: Viruses

Article Title: Effects of Moloney Leukemia Virus 10 Protein on Hepatitis B Virus Infection and Viral Replication

doi: 10.3390/v11070651

Figure Lengend Snippet: Down-regulation of MOV10 (Moloney Leukemia Virus 10) enhances Hepatitis B virus (HBV) gene expression in HepAD38 cells. ( A ) Time course of the experiment. HepAD38 cells were transfected with 50 nM siMOV10 or siControl 24 h before tetracycline (tet) removal. Cells were collected two days after tet removal. ( B ) Western blot showing HBV core and MOV10 expression in siRNA treated cells. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a loading control. ( C ) Quantification of HBV core expression determined by western blotting. ( D ) Intracellular HBV pgRNA level determined by RT-qPCR. Data were normalized to GAPDH mRNA expression. ( E ) Intracellular HBV DNA level determined by qPCR. Data represent mean ± standard deviation from two independent experiments. * p -value < 0.05, two-tailed unpaired t -test.

Article Snippet: The plasmid harboring Cas9, guide-RNA (gRNA), and GFP (pCas9-GFP-Mov10_T1) was purchased from OriGene Technologies.

Techniques: Expressing, Transfection, Western Blot, Quantitative RT-PCR, Standard Deviation, Two Tailed Test

Down-regulation of MOV10 enhances HBV gene expression in HepG2 cells. ( A ) Time course of the experiment. HepG2cells were transfected with 20 nM siMOV10 or siControl 24 h before transfection with pHBV plasmid. Cells were collected two days after HBV plasmid transfection. ( B ) Western blot showing HBV core and MOV10 expression in siRNA treated cells. GAPDH was used as a loading control. ( C ) Quantification of HBV core expression determined by western blotting. ( D ) Intracellular HBV pgRNA level determined by RT-qPCR. Data were normalized to GAPDH mRNA expression. ( E ) Intracellular HBV DNA level determined by qPCR. Data represent mean ± standard deviation from two independent experiments. * p -value < 0.05, two-tailed unpaired t -test.

Journal: Viruses

Article Title: Effects of Moloney Leukemia Virus 10 Protein on Hepatitis B Virus Infection and Viral Replication

doi: 10.3390/v11070651

Figure Lengend Snippet: Down-regulation of MOV10 enhances HBV gene expression in HepG2 cells. ( A ) Time course of the experiment. HepG2cells were transfected with 20 nM siMOV10 or siControl 24 h before transfection with pHBV plasmid. Cells were collected two days after HBV plasmid transfection. ( B ) Western blot showing HBV core and MOV10 expression in siRNA treated cells. GAPDH was used as a loading control. ( C ) Quantification of HBV core expression determined by western blotting. ( D ) Intracellular HBV pgRNA level determined by RT-qPCR. Data were normalized to GAPDH mRNA expression. ( E ) Intracellular HBV DNA level determined by qPCR. Data represent mean ± standard deviation from two independent experiments. * p -value < 0.05, two-tailed unpaired t -test.

Article Snippet: The plasmid harboring Cas9, guide-RNA (gRNA), and GFP (pCas9-GFP-Mov10_T1) was purchased from OriGene Technologies.

Techniques: Expressing, Transfection, Plasmid Preparation, Western Blot, Quantitative RT-PCR, Standard Deviation, Two Tailed Test

HBV gene expression in HepG2 MOV10-KO cells. ( A ) Western blot showing HBV core and MOV10 in HepG2 MOV10-KO cells compared to HepG2 parent cells. GAPDH was used as a loading control. ( B ) Quantification of HBV core expression determined by western blotting. ( C ) Intracellular HBV pgRNA level determined by qPCR after cDNA synthesis. Data were normalized to RPL18A mRNA expression. Data represent mean ± standard deviation from two independent experiments. * p -value < 0.05, two-tailed unpaired t -test.

Journal: Viruses

Article Title: Effects of Moloney Leukemia Virus 10 Protein on Hepatitis B Virus Infection and Viral Replication

doi: 10.3390/v11070651

Figure Lengend Snippet: HBV gene expression in HepG2 MOV10-KO cells. ( A ) Western blot showing HBV core and MOV10 in HepG2 MOV10-KO cells compared to HepG2 parent cells. GAPDH was used as a loading control. ( B ) Quantification of HBV core expression determined by western blotting. ( C ) Intracellular HBV pgRNA level determined by qPCR after cDNA synthesis. Data were normalized to RPL18A mRNA expression. Data represent mean ± standard deviation from two independent experiments. * p -value < 0.05, two-tailed unpaired t -test.

Article Snippet: The plasmid harboring Cas9, guide-RNA (gRNA), and GFP (pCas9-GFP-Mov10_T1) was purchased from OriGene Technologies.

Techniques: Expressing, Western Blot, Standard Deviation, Two Tailed Test

Down-regulation of MOV10 enhances HBV infection of HepG2-sodium taurocholate co-transporting polypeptide (NTCP) cells. HepG2-NTCP-C7 stable cell lines, shControl, and shMOV10, were infected with 250 HBV genome equivalents per cell. Five days later, cells were collected. ( A ) Western blotting showing MOV10 expression in shMOV10 stable cell line compared to shControl. GAPDH was used as a loading control. ( B ) Intracellular HBV pgRNA level determined by RT-qPCR. Data were normalized to GAPDH mRNA expression. ( C ) Intracellular HBV DNA level determined by qPCR. Data represent mean ± standard deviation from at least two independent experiments. * p -value < 0.05, two-tailed unpaired t -test.

Journal: Viruses

Article Title: Effects of Moloney Leukemia Virus 10 Protein on Hepatitis B Virus Infection and Viral Replication

doi: 10.3390/v11070651

Figure Lengend Snippet: Down-regulation of MOV10 enhances HBV infection of HepG2-sodium taurocholate co-transporting polypeptide (NTCP) cells. HepG2-NTCP-C7 stable cell lines, shControl, and shMOV10, were infected with 250 HBV genome equivalents per cell. Five days later, cells were collected. ( A ) Western blotting showing MOV10 expression in shMOV10 stable cell line compared to shControl. GAPDH was used as a loading control. ( B ) Intracellular HBV pgRNA level determined by RT-qPCR. Data were normalized to GAPDH mRNA expression. ( C ) Intracellular HBV DNA level determined by qPCR. Data represent mean ± standard deviation from at least two independent experiments. * p -value < 0.05, two-tailed unpaired t -test.

Article Snippet: The plasmid harboring Cas9, guide-RNA (gRNA), and GFP (pCas9-GFP-Mov10_T1) was purchased from OriGene Technologies.

Techniques: Infection, Stable Transfection, Western Blot, Expressing, Quantitative RT-PCR, Standard Deviation, Two Tailed Test

MOV10 over-expression diminishes HBV infection of HepG2-NTCP cells. HepG2-NTCP-3E8 parent cells and HepG2-NTCP-3E8 HA-MOV10 cells were infected with 400 HBV genome equivalents per cell. Five days later, cells were collected. ( A ) Western blot showing MOV10 expression in 3E8 HA-MOV10 stable cell line compared to HepG2-NTCP-3E8 parent cells. GAPDH was used as a loading control ( B ) Intracellular HBV pgRNA level determined by RT-qPCR. Data were normalized to GAPDH mRNA. ( C ) Intracellular HBV DNA level determined by qPCR. Data represent mean ± standard deviation from two independent experiments. * p -value < 0.05.

Journal: Viruses

Article Title: Effects of Moloney Leukemia Virus 10 Protein on Hepatitis B Virus Infection and Viral Replication

doi: 10.3390/v11070651

Figure Lengend Snippet: MOV10 over-expression diminishes HBV infection of HepG2-NTCP cells. HepG2-NTCP-3E8 parent cells and HepG2-NTCP-3E8 HA-MOV10 cells were infected with 400 HBV genome equivalents per cell. Five days later, cells were collected. ( A ) Western blot showing MOV10 expression in 3E8 HA-MOV10 stable cell line compared to HepG2-NTCP-3E8 parent cells. GAPDH was used as a loading control ( B ) Intracellular HBV pgRNA level determined by RT-qPCR. Data were normalized to GAPDH mRNA. ( C ) Intracellular HBV DNA level determined by qPCR. Data represent mean ± standard deviation from two independent experiments. * p -value < 0.05.

Article Snippet: The plasmid harboring Cas9, guide-RNA (gRNA), and GFP (pCas9-GFP-Mov10_T1) was purchased from OriGene Technologies.

Techniques: Over Expression, Infection, Western Blot, Expressing, Stable Transfection, Quantitative RT-PCR, Standard Deviation

HBV pre-genomic RNA co-immunoprecipitated with MOV10. ( A ) HepG2-NTCP clone 7 cells were infected with HBV and harvested after five days. Immunoprecipitation was performed with an antibody against human MOV10 in the HBV-infected cells and in the non-infected control cells. SYBR Green-based RT-qPCR was done using primers against HBV pre-genomic RNA. ( B ) Human GAPDH mRNA co-immunoprecipitated with MOV10. SYBR Green-based RT-qPCR was done using primers against human GAPDH mRNA. ( n = two independent experiments, ** p = 0.0015, **** p < 0.0001, two-way ANOVA Sidak’s multiple comparisons test).

Journal: Viruses

Article Title: Effects of Moloney Leukemia Virus 10 Protein on Hepatitis B Virus Infection and Viral Replication

doi: 10.3390/v11070651

Figure Lengend Snippet: HBV pre-genomic RNA co-immunoprecipitated with MOV10. ( A ) HepG2-NTCP clone 7 cells were infected with HBV and harvested after five days. Immunoprecipitation was performed with an antibody against human MOV10 in the HBV-infected cells and in the non-infected control cells. SYBR Green-based RT-qPCR was done using primers against HBV pre-genomic RNA. ( B ) Human GAPDH mRNA co-immunoprecipitated with MOV10. SYBR Green-based RT-qPCR was done using primers against human GAPDH mRNA. ( n = two independent experiments, ** p = 0.0015, **** p < 0.0001, two-way ANOVA Sidak’s multiple comparisons test).

Article Snippet: The plasmid harboring Cas9, guide-RNA (gRNA), and GFP (pCas9-GFP-Mov10_T1) was purchased from OriGene Technologies.

Techniques: Immunoprecipitation, Infection, SYBR Green Assay, Quantitative RT-PCR

No specific interaction between MOV10 and HBV core protein. 293T cells were transfected with pC183 and peYFP-MOV10, pC183 and peYFP-empty, peYFP-MOV10 and peYFP-empty, or peYFP-empty only. Where indicated, lysates were pre-treated with RNase A prior to immunoprecipitation with the indicated antibodies. Immunoprecipitates were analyzed by western blotting using the indicated antibodies. ( A ) Proteins in lysates were immunoprecipitated with the anti-HBV core antibody. Immunoprecipitates were analyzed by western blotting using the anti-MOV10 and anti-HBV core antibodies. ( B ) Proteins in lysates were immunoprecipitated with the anti-MOV10 antibody. Immunoprecipitates were analyzed by western blotting using anti-MOV10 and anti-HBV core antibodies. ( C ) Western blotting showing detectable levels of MOV10 and HBV core proteins in tested lysates.

Journal: Viruses

Article Title: Effects of Moloney Leukemia Virus 10 Protein on Hepatitis B Virus Infection and Viral Replication

doi: 10.3390/v11070651

Figure Lengend Snippet: No specific interaction between MOV10 and HBV core protein. 293T cells were transfected with pC183 and peYFP-MOV10, pC183 and peYFP-empty, peYFP-MOV10 and peYFP-empty, or peYFP-empty only. Where indicated, lysates were pre-treated with RNase A prior to immunoprecipitation with the indicated antibodies. Immunoprecipitates were analyzed by western blotting using the indicated antibodies. ( A ) Proteins in lysates were immunoprecipitated with the anti-HBV core antibody. Immunoprecipitates were analyzed by western blotting using the anti-MOV10 and anti-HBV core antibodies. ( B ) Proteins in lysates were immunoprecipitated with the anti-MOV10 antibody. Immunoprecipitates were analyzed by western blotting using anti-MOV10 and anti-HBV core antibodies. ( C ) Western blotting showing detectable levels of MOV10 and HBV core proteins in tested lysates.

Article Snippet: The plasmid harboring Cas9, guide-RNA (gRNA), and GFP (pCas9-GFP-Mov10_T1) was purchased from OriGene Technologies.

Techniques: Transfection, Immunoprecipitation, Western Blot

MOV10 over-expression does not induce the degradation of HBV pgRNA. 293T cells were transfected with HBV core-deficient plasmid (Cp-deficient pHBV) and peYFP-MOV10 or peYFP-empty (eYFP) plasmids. After 48 h, the cells were treated with 5 µg/mL Actinomycin D. The cells were harvested at 0, 2, 4, 6 h after Actinomycin D treatment. Total RNA was extracted and HBV pgRNA levels were determined relative to the 0 h time point. RPL18A mRNA was used as a normalization control. Single phase decay curves are shown. The dotted line represents cells transfected with Cp-deficient pHBV and peYFP in the absence of Actinomycin D. The solid black line represents cells transfected with Cp-deficient pHBV and peYFP in the presence of Actinomycin D. The grey line represents cells transfected with Cp-deficient pHBV and peYFP-MOV10 in the presence of Actinomycin D. Data represent mean ± standard deviation from two independent experiments.

Journal: Viruses

Article Title: Effects of Moloney Leukemia Virus 10 Protein on Hepatitis B Virus Infection and Viral Replication

doi: 10.3390/v11070651

Figure Lengend Snippet: MOV10 over-expression does not induce the degradation of HBV pgRNA. 293T cells were transfected with HBV core-deficient plasmid (Cp-deficient pHBV) and peYFP-MOV10 or peYFP-empty (eYFP) plasmids. After 48 h, the cells were treated with 5 µg/mL Actinomycin D. The cells were harvested at 0, 2, 4, 6 h after Actinomycin D treatment. Total RNA was extracted and HBV pgRNA levels were determined relative to the 0 h time point. RPL18A mRNA was used as a normalization control. Single phase decay curves are shown. The dotted line represents cells transfected with Cp-deficient pHBV and peYFP in the absence of Actinomycin D. The solid black line represents cells transfected with Cp-deficient pHBV and peYFP in the presence of Actinomycin D. The grey line represents cells transfected with Cp-deficient pHBV and peYFP-MOV10 in the presence of Actinomycin D. Data represent mean ± standard deviation from two independent experiments.

Article Snippet: The plasmid harboring Cas9, guide-RNA (gRNA), and GFP (pCas9-GFP-Mov10_T1) was purchased from OriGene Technologies.

Techniques: Over Expression, Transfection, Plasmid Preparation, Standard Deviation

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